Review




Structured Review

Chenomx Inc nmr suite version 10 0 software
Nmr Suite Version 10 0 Software, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pm42116468-116-7-12
Average 86 stars, based on 1 article reviews
nmr suite version 10 0 software - by Bioz Stars, 2026-11
86/100 stars

Images

Related Articles

Nuclear Magnetic Resonance:

Article Title: Aryl hydrocarbon receptor, a promising therapeutic target for ulcerative colitis, impairs HK2-controlled flux of hexosamine-biosynthesis pathway to eliminate NETosis in an N-glycosylation dependent manner.
Article Snippet: .. The collected nuclear magnetic resonance (NMR) spectra were analysed using Chenomx NMR Suite software, and statistical analysis with data visualisation was performed using the MetaboAnalyst platform. .. Statistical analyses were conducted using IBM SPSS Statistics for Windows, version 19.0 (IBM Corp., Armonk, NY, USA).

Article Title: Integrated metabolomic, transcriptomic and network analysis elucidates therapeutic mechanisms of Ganoderma lucidum spore oil against granulomatous pulmonary nodules
Article Snippet: .. The resulting spectra were processed using Chenomx NMR Suite software for Fourier transformation, phase adjustment, baseline correction, and chemical shift calibration. .. Lung tissues were ground after 200 uL of lysis buffer (Beyotime, China) supplemented with protease and phosphatase inhibitor cocktail and then homogenates were incubated on ice for 30 min. Next the concentrations of protein were quantified using the BCA Protein Assay Kit (Beyotime, Shanghai).

Article Title: Metabolomics: Uncovering Insights into Obesity and Diabetes.
Article Snippet: .. After acquiring the NMR spectra, they were processed using the Chenomx NMR suite software, version 9.2. .. Chenomx NMR suite software, version 9.2 (Chenomx Inc., Edmonton, AB, Canada) was used to identify and quantify different groups’ metabolites by identifying the sections of the spectrum (ppm) responsible.

Article Title: Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics
Article Snippet: These quantified data were imported into MATLAB R2015b, where they were organized into a structured data matrix, providing the basis for in-depth multivariate statistical analysis. .. Resonance assignments of metabolites were performed based on the 1D 1H spectra using a combination of the Chenomx NMR Suite software (version 8.3, Chenomx Inc., Edmonton, AB, Canada), the Human Metabolome Database (HMDB, http://www.hmdb.ca/ , accessed on 5 March 2023), and relevant literature. .. The 2D raw spectral data were processed using TopSpin 4.0.6 (Bruker BioSpin, Ettlingen, Germany).

Article Title: Metabolomics Signatures of a Respiratory Tract Infection During an Altitude Training Camp in Elite Rowers.
Article Snippet: .. Each identified bucket (each one corresponding to a VIP) was, if possible, associated with a metabolite based on the current literature and on the database available in Chenomx NMR suite software (Chenomx, Inc., Edmonton, AB, Canada). ..

Article Title: Metabolic response to an acute bout of mild dynamic exercise performed under normobaric moderate hypoxia: A NMR-based metabolomics study.
Article Snippet: .. The assignment of 1H NMR signals was performed using the Chenomx NMR Suite software (version 8.2, Edmonton, Canada, evaluate version), by considering published literature data [23] and the Human Metabolome Database (HMDB, available at http://hmdb.ca) [24]. ..

Article Title: NMR-Based Metabolomics Reveals Position-Specific Signatures Associated with Physical Demands in Professional Soccer Players
Article Snippet: Spectra were processed using TopSpin software (v. 3.6.5, Bruker Corporation, Billerica, MA, USA). .. Metabolite peak identification was performed using Chenomx NMR Suite software (v. 11, Chenomx Inc., Edmonton, AB, Canada) and confirmed against the Human Metabolome Database (HMDB; http://www.hmdb.ca/ , accessed on 1 March 2023). ..

Article Title: Lactate-coated polyurea-siRNA dendriplex: a gene therapy-directed and metabolism-based strategy to impair glioblastoma (GBM).
Article Snippet: Glioblastoma (GBM) is a highly lethal disease with limited treatment options due to its infiltrative nature and the lack of efficient therapy able to cross the protective blood-brain barrier (BBB).. GBMs are metabolically characterized by increased glycolysis and glutamine dependence.. This study explores a novel metabolism-based therapeutic approach using a polyurea generation 4 dendrimer (PUREG4) surface functionalized with lactate (LA) (PUREG4-LA24), to take advantage of glucose-dependent monocarboxylate transporters (MCTs) overexpression, loaded with selenium-chrysin (SeChry) and temozolomide (TMZ) or complexed with anti-glutaminase (GLS1) siRNAs to abrogate glutamine dependence.

Software:

Article Title: Aryl hydrocarbon receptor, a promising therapeutic target for ulcerative colitis, impairs HK2-controlled flux of hexosamine-biosynthesis pathway to eliminate NETosis in an N-glycosylation dependent manner.
Article Snippet: .. The collected nuclear magnetic resonance (NMR) spectra were analysed using Chenomx NMR Suite software, and statistical analysis with data visualisation was performed using the MetaboAnalyst platform. .. Statistical analyses were conducted using IBM SPSS Statistics for Windows, version 19.0 (IBM Corp., Armonk, NY, USA).

Article Title: Integrated metabolomic, transcriptomic and network analysis elucidates therapeutic mechanisms of Ganoderma lucidum spore oil against granulomatous pulmonary nodules
Article Snippet: .. The resulting spectra were processed using Chenomx NMR Suite software for Fourier transformation, phase adjustment, baseline correction, and chemical shift calibration. .. Lung tissues were ground after 200 uL of lysis buffer (Beyotime, China) supplemented with protease and phosphatase inhibitor cocktail and then homogenates were incubated on ice for 30 min. Next the concentrations of protein were quantified using the BCA Protein Assay Kit (Beyotime, Shanghai).

Article Title: Metabolomics: Uncovering Insights into Obesity and Diabetes.
Article Snippet: .. After acquiring the NMR spectra, they were processed using the Chenomx NMR suite software, version 9.2. .. Chenomx NMR suite software, version 9.2 (Chenomx Inc., Edmonton, AB, Canada) was used to identify and quantify different groups’ metabolites by identifying the sections of the spectrum (ppm) responsible.

Article Title: Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics
Article Snippet: These quantified data were imported into MATLAB R2015b, where they were organized into a structured data matrix, providing the basis for in-depth multivariate statistical analysis. .. Resonance assignments of metabolites were performed based on the 1D 1H spectra using a combination of the Chenomx NMR Suite software (version 8.3, Chenomx Inc., Edmonton, AB, Canada), the Human Metabolome Database (HMDB, http://www.hmdb.ca/ , accessed on 5 March 2023), and relevant literature. .. The 2D raw spectral data were processed using TopSpin 4.0.6 (Bruker BioSpin, Ettlingen, Germany).

Article Title: Metabolomics Signatures of a Respiratory Tract Infection During an Altitude Training Camp in Elite Rowers.
Article Snippet: .. Each identified bucket (each one corresponding to a VIP) was, if possible, associated with a metabolite based on the current literature and on the database available in Chenomx NMR suite software (Chenomx, Inc., Edmonton, AB, Canada). ..

Article Title: Metabolic response to an acute bout of mild dynamic exercise performed under normobaric moderate hypoxia: A NMR-based metabolomics study.
Article Snippet: .. The assignment of 1H NMR signals was performed using the Chenomx NMR Suite software (version 8.2, Edmonton, Canada, evaluate version), by considering published literature data [23] and the Human Metabolome Database (HMDB, available at http://hmdb.ca) [24]. ..

Article Title: NMR-Based Metabolomics Reveals Position-Specific Signatures Associated with Physical Demands in Professional Soccer Players
Article Snippet: Spectra were processed using TopSpin software (v. 3.6.5, Bruker Corporation, Billerica, MA, USA). .. Metabolite peak identification was performed using Chenomx NMR Suite software (v. 11, Chenomx Inc., Edmonton, AB, Canada) and confirmed against the Human Metabolome Database (HMDB; http://www.hmdb.ca/ , accessed on 1 March 2023). ..

Article Title: Lactate-coated polyurea-siRNA dendriplex: a gene therapy-directed and metabolism-based strategy to impair glioblastoma (GBM).
Article Snippet: Glioblastoma (GBM) is a highly lethal disease with limited treatment options due to its infiltrative nature and the lack of efficient therapy able to cross the protective blood-brain barrier (BBB).. GBMs are metabolically characterized by increased glycolysis and glutamine dependence.. This study explores a novel metabolism-based therapeutic approach using a polyurea generation 4 dendrimer (PUREG4) surface functionalized with lactate (LA) (PUREG4-LA24), to take advantage of glucose-dependent monocarboxylate transporters (MCTs) overexpression, loaded with selenium-chrysin (SeChry) and temozolomide (TMZ) or complexed with anti-glutaminase (GLS1) siRNAs to abrogate glutamine dependence.

Transformation Assay:

Article Title: Integrated metabolomic, transcriptomic and network analysis elucidates therapeutic mechanisms of Ganoderma lucidum spore oil against granulomatous pulmonary nodules
Article Snippet: .. The resulting spectra were processed using Chenomx NMR Suite software for Fourier transformation, phase adjustment, baseline correction, and chemical shift calibration. .. Lung tissues were ground after 200 uL of lysis buffer (Beyotime, China) supplemented with protease and phosphatase inhibitor cocktail and then homogenates were incubated on ice for 30 min. Next the concentrations of protein were quantified using the BCA Protein Assay Kit (Beyotime, Shanghai).



Similar Products

86
Chenomx Inc nmr suite version 10 0 software
Nmr Suite Version 10 0 Software, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pm42116468-116-7-12
Average 86 stars, based on 1 article reviews
nmr suite version 10 0 software - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

86
Chenomx Inc deconvolution software chenomx nmr suite
Deconvolution Software Chenomx Nmr Suite, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pmc13065565-92-12-14
Average 86 stars, based on 1 article reviews
deconvolution software chenomx nmr suite - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

86
Chenomx Inc nmr suite 9 0 software
Nmr Suite 9 0 Software, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pmc13065565-89-14-18
Average 86 stars, based on 1 article reviews
nmr suite 9 0 software - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

86
Chenomx Inc nmr suite 8 1 software
Nmr Suite 8 1 Software, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pm41941905-114-15-14
Average 86 stars, based on 1 article reviews
nmr suite 8 1 software - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

86
Chenomx Inc chenomx nmr suite software
Glucose metabolism is the main pathway for aryl hydrocarbon receptor (AhR) activation to down-regulate the neutrophil elastase (NE) activity. (a) The diagram of glucose metabolism. (b-e) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of lipopolysaccharide (LPS, 10 μg/mL) or phorbol-12-myristate-13-acetate (PMA, 50 nM). The levels of Magt1, Magt 2, Magt 4, Magt5 and glutamine-fructose-6-phosphate transaminase (GFPT) were detected. (f-j) The neutrophils were treated with FICZ (100 nM) at the present or absence of LPS (10 μg/mL), and the intracellular metabolites were detected using a high-resolution <t>nuclear</t> <t>magnetic</t> resonance <t>(NMR).</t> The results were analyzed by PCA (f) , PLS (g) , OPLS (h) , KEGG enrichment analysis (i) and cluster analysis (j) . (k, l) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM). The relative glucose uptake (k) and intracellular pH values (l) were determined. (m, n) The neutrophils were treated with FICZ (100 nM) or DIM (10 μM) alone or in combination with L-sodium lactate (20 mM), ATP (100 μM) or GlcNAc (20 mM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM), and the release of dsDNA (m) and NE activity (n) was determined. The data were presented as the means ± S.E.M. of three or five independent experiments. ## p < 0.01 vs. Control group; * p < 0.05, ** p < 0.01 vs. LPS group; $$ p < 0.01 vs. LPS + FICZ group; ++ p < 0.01 vs. LPS + DIM group.
Chenomx Nmr Suite Software, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pmc13000937-58-10-10
Average 86 stars, based on 1 article reviews
chenomx nmr suite software - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

86
Chenomx Inc nmr suite software version 9 02
Glucose metabolism is the main pathway for aryl hydrocarbon receptor (AhR) activation to down-regulate the neutrophil elastase (NE) activity. (a) The diagram of glucose metabolism. (b-e) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of lipopolysaccharide (LPS, 10 μg/mL) or phorbol-12-myristate-13-acetate (PMA, 50 nM). The levels of Magt1, Magt 2, Magt 4, Magt5 and glutamine-fructose-6-phosphate transaminase (GFPT) were detected. (f-j) The neutrophils were treated with FICZ (100 nM) at the present or absence of LPS (10 μg/mL), and the intracellular metabolites were detected using a high-resolution <t>nuclear</t> <t>magnetic</t> resonance <t>(NMR).</t> The results were analyzed by PCA (f) , PLS (g) , OPLS (h) , KEGG enrichment analysis (i) and cluster analysis (j) . (k, l) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM). The relative glucose uptake (k) and intracellular pH values (l) were determined. (m, n) The neutrophils were treated with FICZ (100 nM) or DIM (10 μM) alone or in combination with L-sodium lactate (20 mM), ATP (100 μM) or GlcNAc (20 mM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM), and the release of dsDNA (m) and NE activity (n) was determined. The data were presented as the means ± S.E.M. of three or five independent experiments. ## p < 0.01 vs. Control group; * p < 0.05, ** p < 0.01 vs. LPS group; $$ p < 0.01 vs. LPS + FICZ group; ++ p < 0.01 vs. LPS + DIM group.
Nmr Suite Software Version 9 02, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pm41933777-136-1-0
Average 86 stars, based on 1 article reviews
nmr suite software version 9 02 - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

86
Chenomx Inc nmr suite 9 4 software
Glucose metabolism is the main pathway for aryl hydrocarbon receptor (AhR) activation to down-regulate the neutrophil elastase (NE) activity. (a) The diagram of glucose metabolism. (b-e) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of lipopolysaccharide (LPS, 10 μg/mL) or phorbol-12-myristate-13-acetate (PMA, 50 nM). The levels of Magt1, Magt 2, Magt 4, Magt5 and glutamine-fructose-6-phosphate transaminase (GFPT) were detected. (f-j) The neutrophils were treated with FICZ (100 nM) at the present or absence of LPS (10 μg/mL), and the intracellular metabolites were detected using a high-resolution <t>nuclear</t> <t>magnetic</t> resonance <t>(NMR).</t> The results were analyzed by PCA (f) , PLS (g) , OPLS (h) , KEGG enrichment analysis (i) and cluster analysis (j) . (k, l) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM). The relative glucose uptake (k) and intracellular pH values (l) were determined. (m, n) The neutrophils were treated with FICZ (100 nM) or DIM (10 μM) alone or in combination with L-sodium lactate (20 mM), ATP (100 μM) or GlcNAc (20 mM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM), and the release of dsDNA (m) and NE activity (n) was determined. The data were presented as the means ± S.E.M. of three or five independent experiments. ## p < 0.01 vs. Control group; * p < 0.05, ** p < 0.01 vs. LPS group; $$ p < 0.01 vs. LPS + FICZ group; ++ p < 0.01 vs. LPS + DIM group.
Nmr Suite 9 4 Software, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pm41849097-83-9-8
Average 86 stars, based on 1 article reviews
nmr suite 9 4 software - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

86
Chenomx Inc nmr suite software version 8 3
Glucose metabolism is the main pathway for aryl hydrocarbon receptor (AhR) activation to down-regulate the neutrophil elastase (NE) activity. (a) The diagram of glucose metabolism. (b-e) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of lipopolysaccharide (LPS, 10 μg/mL) or phorbol-12-myristate-13-acetate (PMA, 50 nM). The levels of Magt1, Magt 2, Magt 4, Magt5 and glutamine-fructose-6-phosphate transaminase (GFPT) were detected. (f-j) The neutrophils were treated with FICZ (100 nM) at the present or absence of LPS (10 μg/mL), and the intracellular metabolites were detected using a high-resolution <t>nuclear</t> <t>magnetic</t> resonance <t>(NMR).</t> The results were analyzed by PCA (f) , PLS (g) , OPLS (h) , KEGG enrichment analysis (i) and cluster analysis (j) . (k, l) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM). The relative glucose uptake (k) and intracellular pH values (l) were determined. (m, n) The neutrophils were treated with FICZ (100 nM) or DIM (10 μM) alone or in combination with L-sodium lactate (20 mM), ATP (100 μM) or GlcNAc (20 mM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM), and the release of dsDNA (m) and NE activity (n) was determined. The data were presented as the means ± S.E.M. of three or five independent experiments. ## p < 0.01 vs. Control group; * p < 0.05, ** p < 0.01 vs. LPS group; $$ p < 0.01 vs. LPS + FICZ group; ++ p < 0.01 vs. LPS + DIM group.
Nmr Suite Software Version 8 3, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pmc12949077-174-2-7
Average 86 stars, based on 1 article reviews
nmr suite software version 8 3 - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

86
Chenomx Inc nmr suite v11 software
Glucose metabolism is the main pathway for aryl hydrocarbon receptor (AhR) activation to down-regulate the neutrophil elastase (NE) activity. (a) The diagram of glucose metabolism. (b-e) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of lipopolysaccharide (LPS, 10 μg/mL) or phorbol-12-myristate-13-acetate (PMA, 50 nM). The levels of Magt1, Magt 2, Magt 4, Magt5 and glutamine-fructose-6-phosphate transaminase (GFPT) were detected. (f-j) The neutrophils were treated with FICZ (100 nM) at the present or absence of LPS (10 μg/mL), and the intracellular metabolites were detected using a high-resolution <t>nuclear</t> <t>magnetic</t> resonance <t>(NMR).</t> The results were analyzed by PCA (f) , PLS (g) , OPLS (h) , KEGG enrichment analysis (i) and cluster analysis (j) . (k, l) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM). The relative glucose uptake (k) and intracellular pH values (l) were determined. (m, n) The neutrophils were treated with FICZ (100 nM) or DIM (10 μM) alone or in combination with L-sodium lactate (20 mM), ATP (100 μM) or GlcNAc (20 mM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM), and the release of dsDNA (m) and NE activity (n) was determined. The data were presented as the means ± S.E.M. of three or five independent experiments. ## p < 0.01 vs. Control group; * p < 0.05, ** p < 0.01 vs. LPS group; $$ p < 0.01 vs. LPS + FICZ group; ++ p < 0.01 vs. LPS + DIM group.
Nmr Suite V11 Software, supplied by Chenomx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmr+suite+software/chenomx+nmr+suite/pm41681551-107-9-13
Average 86 stars, based on 1 article reviews
nmr suite v11 software - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

Image Search Results


Glucose metabolism is the main pathway for aryl hydrocarbon receptor (AhR) activation to down-regulate the neutrophil elastase (NE) activity. (a) The diagram of glucose metabolism. (b-e) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of lipopolysaccharide (LPS, 10 μg/mL) or phorbol-12-myristate-13-acetate (PMA, 50 nM). The levels of Magt1, Magt 2, Magt 4, Magt5 and glutamine-fructose-6-phosphate transaminase (GFPT) were detected. (f-j) The neutrophils were treated with FICZ (100 nM) at the present or absence of LPS (10 μg/mL), and the intracellular metabolites were detected using a high-resolution nuclear magnetic resonance (NMR). The results were analyzed by PCA (f) , PLS (g) , OPLS (h) , KEGG enrichment analysis (i) and cluster analysis (j) . (k, l) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM). The relative glucose uptake (k) and intracellular pH values (l) were determined. (m, n) The neutrophils were treated with FICZ (100 nM) or DIM (10 μM) alone or in combination with L-sodium lactate (20 mM), ATP (100 μM) or GlcNAc (20 mM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM), and the release of dsDNA (m) and NE activity (n) was determined. The data were presented as the means ± S.E.M. of three or five independent experiments. ## p < 0.01 vs. Control group; * p < 0.05, ** p < 0.01 vs. LPS group; $$ p < 0.01 vs. LPS + FICZ group; ++ p < 0.01 vs. LPS + DIM group.

Journal: Journal of Advanced Research

Article Title: Aryl hydrocarbon receptor impairs HK2-controlled flux of the hexosamine biosynthesis pathway to suppress NETosis in an N -glycosylation-dependent manner

doi: 10.1016/j.jare.2025.06.078

Figure Lengend Snippet: Glucose metabolism is the main pathway for aryl hydrocarbon receptor (AhR) activation to down-regulate the neutrophil elastase (NE) activity. (a) The diagram of glucose metabolism. (b-e) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of lipopolysaccharide (LPS, 10 μg/mL) or phorbol-12-myristate-13-acetate (PMA, 50 nM). The levels of Magt1, Magt 2, Magt 4, Magt5 and glutamine-fructose-6-phosphate transaminase (GFPT) were detected. (f-j) The neutrophils were treated with FICZ (100 nM) at the present or absence of LPS (10 μg/mL), and the intracellular metabolites were detected using a high-resolution nuclear magnetic resonance (NMR). The results were analyzed by PCA (f) , PLS (g) , OPLS (h) , KEGG enrichment analysis (i) and cluster analysis (j) . (k, l) The neutrophils were treated with FICZ (100 nM), DIM (10 μM) or CH223191 (10 μM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM). The relative glucose uptake (k) and intracellular pH values (l) were determined. (m, n) The neutrophils were treated with FICZ (100 nM) or DIM (10 μM) alone or in combination with L-sodium lactate (20 mM), ATP (100 μM) or GlcNAc (20 mM) at the present or absence of LPS (10 μg/mL) or PMA (50 nM), and the release of dsDNA (m) and NE activity (n) was determined. The data were presented as the means ± S.E.M. of three or five independent experiments. ## p < 0.01 vs. Control group; * p < 0.05, ** p < 0.01 vs. LPS group; $$ p < 0.01 vs. LPS + FICZ group; ++ p < 0.01 vs. LPS + DIM group.

Article Snippet: The collected nuclear magnetic resonance (NMR) spectra were analysed using Chenomx NMR Suite software, and statistical analysis with data visualisation was performed using the MetaboAnalyst platform.

Techniques: Activation Assay, Activity Assay, Nuclear Magnetic Resonance, Control